Fig 1: Effects of WNT6 transfection on the development of ovine UGs organoid. (A) Effects of WNT6 suppression (si-WNT6) on organoid development. (B) Effects of WNT6 overexpression (pEX-4-WNT6) on organoid development. (C) The expression of glandular development related genes in UGs organoid by WNT6 suppression. (D) The expression of glandular development related genes in UGs organoid by WNT6 overexpression. NC, Negative control was the siRNA with scrambled sequence absent in the sheep genome. si-WNT6, the small interfering RNA for WNT6. pEX-4-NC, Negative control of pEX-4 was the empty vector. pEX-4-WNT6, pEX-4 vector with WNT6-CDs inserted. Values are presented as the mean ± SDs. Means with different letters indicate significant differences (p < 0.05). Bar = 50 µm.
Fig 2: The effect of WNT6 transfection on cell cycle of EECs. (A,C) Analysis of cell cycle by flow cytometry. (B,D) The percentages of G0/G1, S, and G2/M phases of the cell cycle. (E,F) Effects of WNT6 on protein expression related cycle of EECs. NC, Negative control was the siRNA with scrambled sequence absent in the sheep genome. si-WNT6, the small interfering RNA for WNT6. pEX-4-NC, Negative control of pEX-4 was the empty vector. pEX-4-WNT6, pEX-4 vector with WNT6-CDs inserted. Values are presented as the mean ± SDs. Means with different letters indicate significant differences (p < 0.05).
Fig 3: The effects of WNT6 transfection on ß-catenin expression in EECs. (A,B) Relative expression of WNT6 and ß-catenin protein to ß-actin by WNT6 knockdown. (C,D) Relative expression of WNT6 and ß-catenin protein to ß-actin by WNT6 overexpression. si-WNT6, the small interfering RNA for WNT6. pEX-4-WNT6, pEX-4 vector with WNT6-CDs inserted. siRNA with scrambled sequence absent in the sheep genome and pEX-4 was severed as negative control for knockdown and overexpression, respectively. The values are presented as means ± SDs. Means with different letters indicate significant differences (p < 0.05).
Supplier Page from Abcam for Anti-Wnt6 antibody [EPR9244]